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Cyanobacterial bloom-associated lipopolysaccharides induce pro-inflammatory processes in keratinocytes in vitro

P. Raptová, V. Skočková, P. Babica, I. Sovadinová, E. Sychrová, P. Vídeňská, P. Šplíchalová, O. Vašíček, L. Šindlerová

. 2024 ; 105 (-) : 104342. [pub] 20231212

Language English Country Netherlands

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc24007744

Our previous studies have shown that CyanoHAB LPS (lipopolysaccharides) and LPS from cyanobacterial cultures induce pro-inflammatory effects on intestinal epithelial and immune cells in vitro. To expand our understanding, we investigated their impact on human keratinocytes, which are targeted during water recreational activities. LPS samples were isolated from CyanoHAB biomasses dominated by Microcystis, Aphanizomenon, Planktothrix, and Dolichospermum, or from axenic cultures of these genera. We identified two CyanoHAB biomasses containing a high proportion of Gram-negative bacteria, including potentially pathogenic genera. These biomasses showed the highest induction of interleukin (IL) 8, IL-6, C-C motif chemokine ligand (CCL) 2 (also known as MCP-1), and CCL20 production by HaCaT cells. Interestingly, all CyanoHAB-derived LPS and LPS from axenic cultures (except for Microcystis) accelerated cell proliferation and migration. Our findings highlight the role of G- bacteria composition and LPS structural disparities in influencing these effects, with implications for skin health during recreational activities.

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$a Raptová, P $u Department of Biophysics of Immune System, Institute of Biophysics of the Czech Academy of Sciences, Brno 61200, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno 62500, Czech Republic. Electronic address: raptova@ibp.cz
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$a Cyanobacterial bloom-associated lipopolysaccharides induce pro-inflammatory processes in keratinocytes in vitro / $c P. Raptová, V. Skočková, P. Babica, I. Sovadinová, E. Sychrová, P. Vídeňská, P. Šplíchalová, O. Vašíček, L. Šindlerová
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$a Our previous studies have shown that CyanoHAB LPS (lipopolysaccharides) and LPS from cyanobacterial cultures induce pro-inflammatory effects on intestinal epithelial and immune cells in vitro. To expand our understanding, we investigated their impact on human keratinocytes, which are targeted during water recreational activities. LPS samples were isolated from CyanoHAB biomasses dominated by Microcystis, Aphanizomenon, Planktothrix, and Dolichospermum, or from axenic cultures of these genera. We identified two CyanoHAB biomasses containing a high proportion of Gram-negative bacteria, including potentially pathogenic genera. These biomasses showed the highest induction of interleukin (IL) 8, IL-6, C-C motif chemokine ligand (CCL) 2 (also known as MCP-1), and CCL20 production by HaCaT cells. Interestingly, all CyanoHAB-derived LPS and LPS from axenic cultures (except for Microcystis) accelerated cell proliferation and migration. Our findings highlight the role of G- bacteria composition and LPS structural disparities in influencing these effects, with implications for skin health during recreational activities.
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$a Skočková, V $u Department of Biophysics of Immune System, Institute of Biophysics of the Czech Academy of Sciences, Brno 61200, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno 62500, Czech Republic. Electronic address: skockova@ibp.cz
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$a Babica, P $u RECETOX, Faculty of Science, Masaryk University, Brno 62500, Czech Republic; Department of Experimental Phycology and Ecotoxicology, Institute of Botany of the Czech Academy of Sciences, Brno 60200, Czech Republic. Electronic address: pavel.babica@recetox.muni.cz
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$a Sovadinová, I $u RECETOX, Faculty of Science, Masaryk University, Brno 62500, Czech Republic. Electronic address: sovadinova@recetox.muni.cz
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$a Sychrová, E $u RECETOX, Faculty of Science, Masaryk University, Brno 62500, Czech Republic. Electronic address: eliska.sychrova@recetox.muni.cz
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$a Vídeňská, P $u RECETOX, Faculty of Science, Masaryk University, Brno 62500, Czech Republic. Electronic address: petra.videnska@recetox.muni.cz
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$a Šplíchalová, P $u RECETOX, Faculty of Science, Masaryk University, Brno 62500, Czech Republic. Electronic address: petra.splichalova@recetox.muni.cz
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$a Vašíček, O $u Department of Biophysics of Immune System, Institute of Biophysics of the Czech Academy of Sciences, Brno 61200, Czech Republic. Electronic address: ondrej.vasicek@ibp.cz
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$a Šindlerová, L $u Department of Biophysics of Immune System, Institute of Biophysics of the Czech Academy of Sciences, Brno 61200, Czech Republic. Electronic address: sindler@ibp.cz
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