Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Collaborative exercise: analysis of age estimation using a QIAGEN protocol and the PyroMark Q48 platform

ML. Kampmann, J. Fleckhaus, C. Børsting, H. Jurtikova, A. Piters, J. Papin, Q. Gauthier, M. Ghemrawi, C. Doutremepuich, B. McCord, PM. Schneider, J. Drabek, N. Morling

. 2024 ; 9 (1) : owad055. [pub] 20240105

Status not-indexed Language English Country England, Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc24013142

UNLABELLED: Human age estimation from trace samples may give important leads early in a police investigation by contributing to the description of the perpetrator. Several molecular biomarkers are available for the estimation of chronological age, and currently, DNA methylation patterns are the most promising. In this study, a QIAGEN age protocol for age estimation was tested by five forensic genetic laboratories. The assay comprised bisulfite treatment of the extracted DNA, amplification of five CpG loci (in the genes of ELOVL2, C1orf132, TRIM59, KLF14, and FHL2), and sequencing of the amplicons using the PyroMark Q48 platform. Blood samples from 49 individuals with ages ranging from 18 to 64 years as well as negative and methylation controls were analyzed. An existing age estimation model was applied to display a mean absolute deviation of 3.62 years within the reference data set. KEY POINTS: Age determination as an intelligence tool during investigations can be a powerful tool in forensic genetics.In this study, five laboratories ran 49 samples and obtained a mean absolute deviation of 3.62 years.Five markers were analyzed on a PyroMark Q48 platform.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc24013142
003      
CZ-PrNML
005      
20241008105655.0
007      
ta
008      
240723s2024 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/fsr/owad055 $2 doi
035    __
$a (PubMed)38567377
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Kampmann, Marie-Louise $u Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of CopenhagenCopenhagen, Denmark
245    10
$a Collaborative exercise: analysis of age estimation using a QIAGEN protocol and the PyroMark Q48 platform / $c ML. Kampmann, J. Fleckhaus, C. Børsting, H. Jurtikova, A. Piters, J. Papin, Q. Gauthier, M. Ghemrawi, C. Doutremepuich, B. McCord, PM. Schneider, J. Drabek, N. Morling
520    9_
$a UNLABELLED: Human age estimation from trace samples may give important leads early in a police investigation by contributing to the description of the perpetrator. Several molecular biomarkers are available for the estimation of chronological age, and currently, DNA methylation patterns are the most promising. In this study, a QIAGEN age protocol for age estimation was tested by five forensic genetic laboratories. The assay comprised bisulfite treatment of the extracted DNA, amplification of five CpG loci (in the genes of ELOVL2, C1orf132, TRIM59, KLF14, and FHL2), and sequencing of the amplicons using the PyroMark Q48 platform. Blood samples from 49 individuals with ages ranging from 18 to 64 years as well as negative and methylation controls were analyzed. An existing age estimation model was applied to display a mean absolute deviation of 3.62 years within the reference data set. KEY POINTS: Age determination as an intelligence tool during investigations can be a powerful tool in forensic genetics.In this study, five laboratories ran 49 samples and obtained a mean absolute deviation of 3.62 years.Five markers were analyzed on a PyroMark Q48 platform.
590    __
$a NEINDEXOVÁNO
655    _2
$a časopisecké články $7 D016428
700    1_
$a Fleckhaus, Jan $u Institute of Legal Medicine, Faculty of Medicine and University Clinic, University of Cologne, Cologne, Germany
700    1_
$a Børsting, Claus $u Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of CopenhagenCopenhagen, Denmark
700    1_
$a Jurtíková, Helena $u Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc and the University Hospital Olomouc, Olomouc, the Czech Republic $7 xx0323107
700    1_
$a Piters, Alice $u Laboratoire d'Hématologie Médico-Légale, Bordeaux Cedex, France
700    1_
$a Papin, Julien $u Laboratoire d'Hématologie Médico-Légale, Bordeaux Cedex, France
700    1_
$a Gauthier, Quentin $u Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA
700    1_
$a Ghemrawi, Mirna $u Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA
700    1_
$a Doutremepuich, Christian $u Laboratoire d'Hématologie Médico-Légale, Bordeaux Cedex, France
700    1_
$a McCord, Bruce $u Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA
700    1_
$a Schneider, Peter M $u Institute of Legal Medicine, Faculty of Medicine and University Clinic, University of Cologne, Cologne, Germany
700    1_
$a Drabek, Jiri $u Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc and the University Hospital Olomouc, Olomouc, the Czech Republic
700    1_
$a Morling, Niels $u Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of CopenhagenCopenhagen, Denmark
773    0_
$w MED00205375 $t Forensic sciences research $x 2471-1411 $g Roč. 9, č. 1 (2024), s. owad055
856    41
$u https://pubmed.ncbi.nlm.nih.gov/38567377 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20240723 $b ABA008
991    __
$a 20241008105652 $b ABA008
999    __
$a ok $b bmc $g 2125616 $s 1225005
BAS    __
$a 3
BAS    __
$a PreBMC-PubMed-not-MEDLINE
BMC    __
$a 2024 $b 9 $c 1 $d owad055 $e 20240105 $i 2471-1411 $m Forensic sciences research $n Forensic Sci Res $x MED00205375
LZP    __
$a Pubmed-20240723

Find record

Citation metrics

Archiving options