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Fecal tryptophan metabolite profiling in newborns in relation to microbiota and antibiotic treatment
AC. Aust, V. Vidova, K. Coufalikova, S. Smetanova, K. Kozeluhova, L. Micenkova, P. Videnska, S. Smatana, E. Budinska, I. Borek, P. Janku, J. Klanova, Z. Spacil, V. Thon
Language English Country Germany
Document type Journal Article
Grant support
CZ.02.1.01/0.0/ 0.0/15_003/0000469
Ministerstvo Školství, Mládeže a Tělovýchovy
LM2023069
Ministerstvo Školství, Mládeže a Tělovýchovy
65269705
Ministerstvo Školství, Mládeže a Tělovýchovy
LM2023069
Ministerstvo Školství, Mládeže a Tělovýchovy
CZ.02.1.01/0.0/ 0.0/15_003/0000469
Ministerstvo Školství, Mládeže a Tělovýchovy
65269705
Ministerstvo Zdravotnictví Ceské Republiky
857560
HORIZON EUROPE Research Infrastructures
NLK
ProQuest Central
from 1997-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 1999-12-01 to 1 year ago
Health & Medicine (ProQuest)
from 1997-01-01 to 1 year ago
Springer Journals Complete - Open Access
from 2024-12-01
Springer Nature OA/Free Journals
from 2024-12-01
- MeSH
- Anti-Bacterial Agents * MeSH
- Bifidobacterium metabolism growth & development MeSH
- Feces * microbiology chemistry MeSH
- Indoles metabolism MeSH
- Indoleacetic Acids metabolism MeSH
- Humans MeSH
- Longitudinal Studies MeSH
- Meconium * microbiology chemistry MeSH
- Infant, Newborn MeSH
- RNA, Ribosomal, 16S * genetics MeSH
- Gastrointestinal Microbiome * drug effects MeSH
- Tryptophan * metabolism MeSH
- Chromatography, High Pressure Liquid MeSH
- Check Tag
- Humans MeSH
- Infant, Newborn MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
In the first days of life, the newborns' intestinal microbiota develops simultaneously with the intestinal gut barrier and follows intestinal immunity. The mode of delivery shows significant impact on microbial development and, thus, the initiation of the tryptophan catabolism pathway. Further antibiotics (ATB) treatment of mothers before or during delivery affects the microbial and tryptophan metabolite composition of stool of the caesarean- and vaginal-delivered newborns. The determination of microbiome and levels of tryptophan microbial metabolites in meconium and stool can characterize intestinal colonization of a newborn. From 134 samples from the Central European Longitudinal Studies of Parents and Children: The Next Generation (CELSPAC: TNG) cohort study, 16S rRNA gene sequencing was performed, and microbial tryptophan metabolites were quantified using ultra-high-performance liquid chromatography with triple-quadrupole mass spectrometry. Microbial diversity and concentrations of tryptophan metabolites were significantly higher in stool compared to meconium. Treatment of mothers with ATB before or during delivery affects metabolite composition and microbial diversity in stool of vaginal- and caesarean-delivered newborns. Correlation of microbial and metabolite composition shows significant positive correlations of indol-3-lactic acid, N-acetyl-tryptophan and indol-3-acetic acid with Bifidobacterium, Bacteroides and Peptoclostridium. The positive effect of vaginal delivery on newborns' microbiome development is degraded when mother is treated with ATB before or during delivery. KEY POINTS: • Antibiotic treatment diminishes the positive effects of vaginal delivery. • Antibiotic treatment affects metabolite and microbial composition in newborns. • Bifidobacterium and Peptoclostridium could be the producer of indole-lactic acid.
Clinic of Gynecology and Obstetrics University Hospital Brno Brno Czech Republic
Department of Neonatology University Hospital Brno Brno Czech Republic
References provided by Crossref.org
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- $a In the first days of life, the newborns' intestinal microbiota develops simultaneously with the intestinal gut barrier and follows intestinal immunity. The mode of delivery shows significant impact on microbial development and, thus, the initiation of the tryptophan catabolism pathway. Further antibiotics (ATB) treatment of mothers before or during delivery affects the microbial and tryptophan metabolite composition of stool of the caesarean- and vaginal-delivered newborns. The determination of microbiome and levels of tryptophan microbial metabolites in meconium and stool can characterize intestinal colonization of a newborn. From 134 samples from the Central European Longitudinal Studies of Parents and Children: The Next Generation (CELSPAC: TNG) cohort study, 16S rRNA gene sequencing was performed, and microbial tryptophan metabolites were quantified using ultra-high-performance liquid chromatography with triple-quadrupole mass spectrometry. Microbial diversity and concentrations of tryptophan metabolites were significantly higher in stool compared to meconium. Treatment of mothers with ATB before or during delivery affects metabolite composition and microbial diversity in stool of vaginal- and caesarean-delivered newborns. Correlation of microbial and metabolite composition shows significant positive correlations of indol-3-lactic acid, N-acetyl-tryptophan and indol-3-acetic acid with Bifidobacterium, Bacteroides and Peptoclostridium. The positive effect of vaginal delivery on newborns' microbiome development is degraded when mother is treated with ATB before or during delivery. KEY POINTS: • Antibiotic treatment diminishes the positive effects of vaginal delivery. • Antibiotic treatment affects metabolite and microbial composition in newborns. • Bifidobacterium and Peptoclostridium could be the producer of indole-lactic acid.
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