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Fiber-type composition and 3D capillary analysis of the human splenius capitis muscle
N. Pollak, CK. Ugwoke, N. Umek, A. Alibegović, J. Janáček, B. Radochová, E. Cvetko
Jazyk angličtina Země Bosna a Hercegovina
Typ dokumentu časopisecké články
NLK
Directory of Open Access Journals
od 2023
PubMed Central
od 2023
Medline Complete (EBSCOhost)
od 2023-02-01
ROAD: Directory of Open Access Scholarly Resources
od 2023
PubMed
39340391
DOI
10.17305/bb.2024.10787
Knihovny.cz E-zdroje
- MeSH
- dospělí MeSH
- kapiláry * metabolismus MeSH
- kosterní svalová vlákna * metabolismus MeSH
- krční svaly * krevní zásobení metabolismus MeSH
- lidé MeSH
- těžké řetězce myosinu metabolismus MeSH
- zobrazování trojrozměrné MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Despite the significance of neck muscles in musculoskeletal disorders, their microscopic anatomy remains poorly characterized. This study examined the splenius capitis muscle, focusing on its fiber-type composition, fiber size, and capillary network characteristics. For comparison and validation, the vastus lateralis muscle was also analyzed. Muscle samples from 13 young male subjects (mean age ± SD: 35.7 ± 8.6 years) were collected within 24-h post-mortem during autopsy. Myosin heavy chain (MyHC) isoform expression was characterized immunohistochemically in 10 μm sections, while the capillary network architecture was assessed in 100 μm sections. Immunofluorescence staining, confocal microscopy, and 3D image analysis were employed to quantify capillary tortuosity, anisotropy, branch density (Br dens), and the length of capillaries per muscle volume (LV), per muscle fiber length (LL), per fiber surface area (LS), and per fiber volume (LVf). Compared to the vastus lateralis muscle, the splenius capitis muscle had a higher percentage of type 1 fibers (51.2% vs 39.7%), fewer type 2a fibers (16.2% vs 31.4%), and smaller fiber diameters (35.5-40.9 μm vs 47-56.1 μm). It also displayed lower Br dens (P = 0.0069), higher anisotropy (P = 0.0004), and lower LL (P < 0.0001) but higher LVf (P = 0.0486). In the splenius capitis muscle, body mass index (BMI) negatively correlated with LV (P = 0.0155), LS (P = 0.0091), LVf (P = 0.0137), and anisotropy (P = 0.0425), and positively correlated with tortuosity (P = 0.0473), indicating a reduction in the capillary network. In the vastus lateralis muscle, only LV (P = 0.0161) decreased with high BMI. This study characterized the fiber-type composition, fiber size, and 3D capillary network of the splenius capitis muscle, establishing a baseline for investigations into pathological muscle alterations.
Institute of Anatomy Faculty of Medicine University of Ljubljana Ljubljana Slovenia
Institute of Forensic Medicine Faculty of Medicine University of Ljubljana Ljubljana Slovenia
Citace poskytuje Crossref.org
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