Hybridoma growth and monoclonal antibody production in iron-rich protein-free medium: effect of nutrient concentration
Language English Country United States Media print
Document type Journal Article
PubMed
1367699
DOI
10.1007/bf00135636
Knihovny.cz E-resources
- MeSH
- Cell Division drug effects MeSH
- Hybridomas cytology drug effects immunology MeSH
- Immunoglobulin G biosynthesis MeSH
- Immunoglobulin kappa-Chains biosynthesis MeSH
- Kinetics MeSH
- Culture Media, Serum-Free MeSH
- Culture Techniques methods MeSH
- Humans MeSH
- Antibodies, Monoclonal biosynthesis MeSH
- Mice MeSH
- Thyrotropin immunology MeSH
- Cell Survival drug effects MeSH
- Ferric Compounds pharmacology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- ferric citrate MeSH Browser
- Immunoglobulin G MeSH
- Immunoglobulin kappa-Chains MeSH
- Culture Media, Serum-Free MeSH
- Antibodies, Monoclonal MeSH
- Thyrotropin MeSH
- Ferric Compounds MeSH
The iron-rich (500 microM ferric citrate) protein-free supplement was added to six different basal media. Cell growth and monoclonal antibody production of a mouse-mouse hybridoma were investigated in 1.3 1 batch cultures performed in a laboratory bioreactor with automatic control of pH and dissolved oxygen concentration. RPMI 1640 served as the control medium. Fortification of the basal medium by balanced mixtures of amino acids and vitamins showed higher positive effect than daily supplementation by glucose and glutamine. Strongly fortified medium, based on RPMI 1640, was found superior to other basal media. The viability index increased by a factor of 3.04 and the total antibody production by a factor of 2.82, relative to the control.
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