Spore walls of Backusella lamprospora (Mucorales) were stained with ten fluorescent brighteners (FB) and the intensity of their fluorescence was determined. The fluorescence was most intense with Uvitex 2B (100%), other brighteners yielding lower fluorescence intensities: Blankophor BA 267% and BA 200% about 75%, Rylux BSU about 50%, other Rylux agents 10-30%. The agents most suitable for microscopic diagnostics of human and animal mycoses are Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU, and also Rylux BS and PRS. The regulation of excessive fluorescence of fungal cells during microscopic observation is discussed. For the purposes of microscopic diagnosis of human and animal mycosis Uvitex 2B, Blankophor BA 267% and BA 200%, Rylux BSU and, possibly, Rylux BS and PRS are recommended.

Department of Medical Biology Faculty of Medicine Palacký University Olomouc Czechoslovakia

See more in PubMed

Mykosen. 1987 Jan;30(1):21-4 PubMed

Cesk Patol. 1989 Nov;25(4):244-50 PubMed

Cesk Epidemiol Mikrobiol Imunol. 1989 May;38(3):161-6 PubMed

Rylux BSU stimulates spore germination in Trichophyton mentagrophytes and Aspergillus fumigatus and increases the survival rate after UV-irradiation

Nikkomycin Z counteracts Rylux BSU and Congo red inhibition of Saccharomyces cerevisiae growth but does not prevent formation of aberrant cell walls

The fluorescence brightener Rylux BSU induces dimorphism in Basidiobolus ranarum