Failure of azidothymidine to inhibit human immunodeficiency virus (HIV) replication in a promonocytic cell line (U937)
Language English Country Switzerland Media print
Document type Journal Article
PubMed
1724875
Knihovny.cz E-resources
- MeSH
- Depression, Chemical MeSH
- Lymphoma, Large B-Cell, Diffuse pathology MeSH
- HIV Antigens biosynthesis MeSH
- HIV Reverse Transcriptase MeSH
- HIV-1 drug effects physiology MeSH
- Reverse Transcriptase Inhibitors MeSH
- Humans MeSH
- Monocytes drug effects microbiology MeSH
- Tumor Cells, Cultured drug effects microbiology MeSH
- Virus Replication drug effects MeSH
- Zidovudine pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- HIV Antigens MeSH
- HIV Reverse Transcriptase MeSH
- Reverse Transcriptase Inhibitors MeSH
- Zidovudine MeSH
The inhibitory effect of azidothymidine (AZT) on HIV replication in the promonocytic cell line U937 was investigated. After infection with HIV-1/LAV virus, U937 cells were cultured for prolonged period in the presence of the drug at a final concentrations of 20 mumol/l or 50 mumol/l, respectively. The antiviral activity was determined according to the inhibition of viral reverse transcriptase activity, and of viral antigen production (immunofluorescence assay). We conclude that virus production was not efficiently influenced during long term passage even at high drug concentrations.
