Isolation and characterization of glycocalyx in Streptomyces aureofaciens
Language English Country United States Media print
Document type Journal Article
PubMed
1821871
DOI
10.1007/bf02884067
Knihovny.cz E-resources
- MeSH
- Amino Acids analysis MeSH
- Cell Fractionation MeSH
- Glycoproteins chemistry isolation & purification MeSH
- Polysaccharides chemistry isolation & purification MeSH
- Streptomyces aureofaciens chemistry MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Amino Acids MeSH
- Glycoproteins MeSH
- Polysaccharides MeSH
The surface layer, considered to be glycocalyx according to electron-microscopic observations, was separated from a low-production strain of Streptomyces aureofaciens by solubilization with urea and subsequent sonication. The isolation procedure was developed using various agents; neutral phosphatase served as a marker indicating the amount of the material released. The peripheral structure consisted predominantly of glycoprotein and differed from S-layers.
