Influence of repeated lyophilization on the survival of Deinococcus proteolyticus, Micrococcus luteus and Escherichia coli
Language English Country United States Media print
Document type Comparative Study, Journal Article
PubMed
1841852
DOI
10.1007/bf02935825
Knihovny.cz E-resources
- MeSH
- DNA, Bacterial genetics radiation effects MeSH
- Escherichia coli cytology genetics radiation effects MeSH
- Freeze Drying MeSH
- Micrococcus luteus cytology genetics radiation effects MeSH
- Micrococcus cytology genetics radiation effects MeSH
- Radiation Tolerance MeSH
- Gamma Rays MeSH
- Freezing MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- DNA, Bacterial MeSH
Repeated lyophilization of Deinococcus proteolyticus, Micrococcus luteus and Escherichia coli cells results in a successive decrease of their survival. The survival curve is exponential with E. coli and M. luteus, and sigmoidal with a broad shoulder with D. proteolyticus both after repeated lyophilization and after UV- or gamma-irradiation. When cells were subjected to gamma-irradiation after a 20-fold freeze-drying, the corresponding survival curve became exponential without the shoulder. Hence we assume that irradiation and repeated lyophilization afflict the same cellular structures and/or functions.
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