DNA-dependent RNA polymerase from an extremely thermophilic hydrogen-oxidizing bacterium Calderobacterium hydrogenophilum
Language English Country United States Media print
Document type Journal Article
PubMed
2025266
DOI
10.1016/0006-291x(91)91657-x
PII: 0006-291X(91)91657-X
Knihovny.cz E-resources
- MeSH
- Chromatography, Affinity methods MeSH
- DNA-Directed RNA Polymerases isolation & purification metabolism MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Eubacterium enzymology MeSH
- Templates, Genetic MeSH
- Kinetics MeSH
- Molecular Weight MeSH
- Oxidation-Reduction MeSH
- Plasmids MeSH
- Enzyme Stability MeSH
- Hydrogen metabolism MeSH
- Hot Temperature MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA-Directed RNA Polymerases MeSH
- Hydrogen MeSH
Extremely thermophilic bacterium Calderobacterium hydrogenophilum contains DNA-dependent RNA polymerase with unusual properties. Purified enzyme is thermoresistant (40 min at 100 degrees C) and exhibits similar subunit composition as eubacterial RNA polymerases (e.g. Escherichia coli). However, the enzyme is not susceptible to antibiotics which inhibit eubacterial RNA polymerases (rifampicin and streptolydigin). The activity of the enzyme is inhibited by actinomycin D, daunomycin and heparin.
References provided by Crossref.org
