Nucleosomes occurring in protein-free hybridoma cell culture. Evidence for programmed cell death
Language English Country England, Great Britain Media print
Document type Journal Article
PubMed
2060652
DOI
10.1016/0014-5793(91)80705-8
PII: 0014-5793(91)80705-8
Knihovny.cz E-resources
- MeSH
- Amino Acids analysis MeSH
- DNA analysis metabolism MeSH
- Chromatography, Gel MeSH
- Hybridomas metabolism ultrastructure MeSH
- Cells, Cultured MeSH
- Molecular Weight MeSH
- Mice MeSH
- Nucleoproteins analysis metabolism MeSH
- Nucleosomes metabolism MeSH
- Cell Survival * MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Amino Acids MeSH
- DNA MeSH
- Nucleoproteins MeSH
- Nucleosomes MeSH
In addition to monoclonal immunoglobulin, two kinds of nucleoproteins, NP1 and NP2, were isolated from the supernatants of hybridoma cultures set up in a protein-free medium. As shown by SDS-electrophoresis the two nucleoproteins shared a set of proteins (apparent Mr 11,000 to 15,000), and differed in the DNA moiety (approximately 150 bp in NP1, approximately 350 bp in NP2). The amino acid composition of the protein moiety confirmed the nucleosomal origin of NP1 and NP2. The findings support the view that in hybridoma cultures the cells undergo death by apoptosis, i.e. a programmed process characterized by initial fragmentation of chromatin.
References provided by Crossref.org
Cell suicide in starving hybridoma culture: survival-signal effect of some amino acids
Cell suicide in starving hybridoma culture: survival-signal effect of some amino acids
Fragmented DNA and apoptotic bodies document the programmed way of cell death in hybridoma cultures
