Histologická technika ke znázornĕni cév v cns: impregnace bazálni membrány endotelu podle Gallyase
[Histological technic for imaging the blood vessels in the central nervous system: impregnation of the basal endothelial membrane using the method of Gallyas]
Language Czech Country Czech Republic Media print
Document type English Abstract, Journal Article
PubMed
2130505
- MeSH
- Silver Staining MeSH
- Central Nervous System blood supply MeSH
- Endothelium, Vascular cytology MeSH
- Histological Techniques * MeSH
- Rats MeSH
- Humans MeSH
- Microcirculation cytology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- English Abstract MeSH
- Journal Article MeSH
The authors present their experience on Gallays' silver staining of vascular basal lamina--especially of microcirculary bed--in human and rat brain and spinal cord. They pointed out that the quality of impregnation was strongly influenced by a duration of formol fixation and pyridine senzibilization. When using sections of equal thickness they demonstrated this method offers a more complex angioarchitectonic pattern then India ink injection in vessels' lumina. More over the so called intercapillary bridges including their dynamics, origin and regression are uniquely revealed by this method.