Binding properties of avian retroviral proteins. III. Binding of protein ASLV MA(p19) to viral RNA and proviral DNA
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
2160895
Knihovny.cz E-resources
- MeSH
- DNA-Binding Proteins metabolism MeSH
- DNA, Viral metabolism ultrastructure MeSH
- Cloning, Molecular MeSH
- Chickens MeSH
- Viral Matrix Proteins metabolism ultrastructure MeSH
- Proviruses genetics metabolism MeSH
- Retroviridae Proteins metabolism ultrastructure MeSH
- RNA, Viral metabolism ultrastructure MeSH
- Binding Sites MeSH
- Avian Leukosis Virus genetics MeSH
- Avian Myeloblastosis Virus genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- avian retrovirus proteins MeSH Browser
- DNA-Binding Proteins MeSH
- DNA, Viral MeSH
- Viral Matrix Proteins MeSH
- Retroviridae Proteins MeSH
- RNA, Viral MeSH
The binding of the avian retroviral matrix protein ASLV MA(p19) to homologous viral ssRNA and proviral dsDNA was analysed using electron microscopic methods combined with a special computer evaluation. No binding affinity of MA(p19) to homologous nondenatured or denatured viral RNA was found. In contrast, ASLV MA(p19) was shown to have one specific binding site on MAV-1 proviral dsDNA at position 6795 +/- 345 bp from the 5' end of the molecule. A second specific binding site was found in a cellular sequence.
