RNA and protein synthesis requirements for the resumption of meiosis in rabbit oocytes: the role of cumulus cells
Language English Country France Media print
Document type Journal Article
PubMed
2481457
DOI
10.1051/rnd:19890509
Knihovny.cz E-resources
- MeSH
- Amanitins pharmacology MeSH
- Cycloheximide pharmacology MeSH
- Transcription, Genetic MeSH
- Rabbits * MeSH
- Meiosis * drug effects MeSH
- Oocytes cytology drug effects physiology MeSH
- Ovarian Follicle cytology physiology MeSH
- Protein Biosynthesis * MeSH
- RNA biosynthesis MeSH
- Animals MeSH
- Check Tag
- Rabbits * MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Amanitins MeSH
- Cycloheximide MeSH
- RNA MeSH
In vitro maturation of rabbit cumulus-enclosed oocytes was fully inhibited in alpha-amanitin- (100 micrograms/ml) and cycloheximide- (5 micrograms/ml) supplemented media. The inhibition was reversible and substantially reduced by delaying the addition of alpha-amanitin (2h) or cycloheximide (3 h). In contrast, both drugs did not inhibit germinal vesicle breakdown in denuded oocytes. Co-culture of granulosa cells (1 x 10(6)/ml) with denuded oocytes did not substitute for an intact cumulus. The data presented here suggest that the resumption of meiosis in rabbit cumulus-enclosed oocytes is dependent upon early transcriptional and translational events which probably occur within the cumulus cells.
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