Expression of a bovine leukaemia virus envelope fusion protein in E. coli
Folia biologica.
1989 ;
35 (1) :
35-41.
Language English Country Czech Republic Media print
Document type Journal Article
Persistent link
https://www.medvik.cz/link/pmid02541029
PubMed
2541029
Knihovny.cz E-resources
- MeSH
- Genes, Bacterial MeSH
- Escherichia coli genetics MeSH
- Kidney cytology MeSH
- Sheep MeSH
- Peptides analysis MeSH
- Plasmids MeSH
- Fetus MeSH
- Viral Envelope Proteins genetics MeSH
- DNA, Recombinant MeSH
- Retroviridae genetics MeSH
- Leukemia Virus, Bovine genetics MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Peptides MeSH
- Viral Envelope Proteins MeSH
- DNA, Recombinant MeSH
An expression plasmid containing 1.224 bp of the bovine leukaemia virus (BLV) env gene was constructed. The polypeptides encoded by six recombinant plasmids were analysed by electrophoretic transfer blot analysis. Two new proteins of 150-160 kDa and 60 kDa, respectively, were found in whole cellular extracts using sera of naturally infected cattle and/or by use of mouse monoclonal antibodies against gp51.
