Bacterial expression of the p24 gag protein of the bovine leukaemia virus
Folia biologica.
1989 ;
35 (1) :
42-4.
Language English Country Czech Republic Media print
Document type Journal Article
Persistent link
https://www.medvik.cz/link/pmid02541030
PubMed
2541030
Knihovny.cz E-resources
- MeSH
- Genes, Bacterial MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Escherichia coli genetics MeSH
- Antibodies, Monoclonal MeSH
- Peptides analysis MeSH
- DNA, Recombinant MeSH
- Retroviridae genetics MeSH
- Viral Proteins genetics metabolism MeSH
- Leukemia Virus, Bovine genetics MeSH
- Blotting, Western MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antibodies, Monoclonal MeSH
- Peptides MeSH
- DNA, Recombinant MeSH
- Viral Proteins MeSH
The possibility of expression of the gag gene of bovine leukaemia virus (BLV) in the bacterial system was investigated. The DNA fragment coding for the gag core 24 kDa protein of BLV was inserted into the pORF1 expression vector. The polypeptides expressed in E. coli were analysed by Western blotting. The bacterially synthesized antigens were detected by the serum of a BLV-infected cow and by mouse monoclonal antibodies against the native p24 gag protein.
