Netropsin increases formation of mRNA coding for a neutral metalloproteinase in Bacillus megaterium
Language English Country Germany Media print
Document type Journal Article
- MeSH
- Amino Acids pharmacology MeSH
- Bacillus megaterium drug effects enzymology genetics growth & development physiology MeSH
- Transcription, Genetic drug effects MeSH
- Guanidines pharmacology MeSH
- Culture Media MeSH
- RNA, Messenger biosynthesis MeSH
- Metalloendopeptidases biosynthesis genetics MeSH
- Netropsin pharmacology MeSH
- Half-Life MeSH
- Protein Biosynthesis drug effects MeSH
- Gene Expression Regulation drug effects MeSH
- Spores, Bacterial MeSH
- Temperature MeSH
- Tetracycline pharmacology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Amino Acids MeSH
- Guanidines MeSH
- Culture Media MeSH
- RNA, Messenger MeSH
- Metalloendopeptidases MeSH
- Netropsin MeSH
- Tetracycline MeSH
The anticancer drug netropsin increases the synthesis of an exocellular metalloproteinase during exponential growth as well as in the stationary phase of a sporulating strain of Bacillus megaterium. Its effect is due to a stimulation of the synthesis of the mRNA coding for the proteinase, determined as a residual synthesis of the enzyme in the presence of actinomycin D. The half-life of the proteinase mRNA (5-6 min at 35 degrees C) is not affected by netropsin. Netropsin relieves partially the repression of the proteinase mRNA caused by amino acids, whereas the repression brought about by an increased temperature is almost unaffected by the drug.
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General and molecular microbiology and microbial genetics in the IM CAS
