Production of methionine and glutamic acid from n-alkanes by Serratia marcescens var. kiliensis
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
2872146
DOI
10.1007/bf02926827
Knihovny.cz E-resources
- MeSH
- Alkanes metabolism MeSH
- Time Factors MeSH
- Phosphates metabolism MeSH
- Glutamates biosynthesis MeSH
- Hydrogen-Ion Concentration MeSH
- Culture Media MeSH
- Glutamic Acid MeSH
- Methionine biosynthesis MeSH
- Serratia marcescens growth & development metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alkanes MeSH
- ammonium phosphate MeSH Browser
- Phosphates MeSH
- Glutamates MeSH
- Culture Media MeSH
- Glutamic Acid MeSH
- Methionine MeSH
- n-hexadecane MeSH Browser
A hydrocarbon-utilizing Serratia marcescens var. kiliensis grew and accumulated methionine and glutamic acid in a synthetic medium with hydrocarbon as sole carbon source. n-Hexadecane and ammonium phosphate were found as the most suitable carbon and nitrogen sources, respectively. Optimum pH for growth and methionine production was 7.2, and that for glutamic acid accumulation was 7.4. Yeast extract significantly stimulated growth and amino acid production and could be substituted by cyanocobalamine. Benzylpenicillin, Tween 80, SDS or EDTA did not increase amino acid production. Under optimal cultural conditions in the laboratory the organism produced 1.68 g of glutamic acid and 0.78 g of methionine per litre.
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