First detection of Cryptosporidium sp. oocysts in calf faeces by floatation method
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
7106652
Knihovny.cz E-resources
- MeSH
- Coccidia cytology isolation & purification MeSH
- Feces parasitology MeSH
- Coccidiosis parasitology veterinary MeSH
- Methods MeSH
- Cattle Diseases parasitology MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Oocysts of coccidia of the genes Cryptosporidium were detected for the first time in the faeces of naturally infected calves using centrifugation floatation method with saturated solution of ZnSo4 and Breza's floatation solution. Since the detection of oocysts in the faeces is very difficult, a new method has been prepared. The oocysts recovered from the faeces were sporulated, spherical or slightly elliptical, with a smooth, colourless and thin wall. In the fresh preparation after floatation the oocysts measured 5.4 X 4.6 micrometer on the average, whereas in the smears stained after Giemsa, their average size was 4.8 X 4.3 micrometer. A typical feature of the oocysts is a residual body appearing as a dark point at one pole of the oocyst when observed by optical microscope. The oocyst contains four sporozoites forming a C-shaped rim around the residual body. No sporocysts were recorded. The residual body stains blue and the nucleus dark red by Giemsa's method.