Identification of a cytolytic protein in the coelomic fluid of Eisenia foetida earthworms
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
7622179
DOI
10.1016/0165-2478(94)00248-p
PII: 0165-2478(94)00248-P
Knihovny.cz E-resources
- MeSH
- Chromatography, Affinity MeSH
- Cytotoxins immunology isolation & purification metabolism pharmacology MeSH
- Fibrosarcoma pathology MeSH
- Hemolytic Plaque Technique MeSH
- Carcinoma pathology MeSH
- Lectins * MeSH
- Antibodies, Monoclonal immunology MeSH
- Mice MeSH
- Tumor Cells, Cultured MeSH
- Lung Neoplasms pathology MeSH
- Oligochaeta chemistry MeSH
- Opsonin Proteins metabolism MeSH
- Sheep blood MeSH
- Flow Cytometry MeSH
- Body Fluids chemistry MeSH
- Cross Reactions MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- coelomic cytolytic factor 1, Eisenia fetida MeSH Browser
- Cytotoxins MeSH
- Lectins * MeSH
- Antibodies, Monoclonal MeSH
- Opsonin Proteins MeSH
Total coelomic fluid of earthworms Eisenia foetida (Oligochaeta, Annelida) is capable of lysing different mammalian tumor cell lines. This cytolytic activity is different from tumor necrosis factor (TNF)-mediated lysis and is not due to proteolysis. Total coelomic fluid was subjected to ion-exchange chromatography separation and a fraction with prominent cytolytic activity was used to elicit monoclonal antibodies that were screened for their capacity to neutralize the cytolytic effect of total coelomic fluid. One of the prepared neutralizing IgG antibodies was used for the immunoaffinity purification of a cytolytic factor from total coelomic fluid. SDS-PAGE and Western blot analyses revealed a protein band with an apparent molecular weight of 42 kDa. This cytolytic protein (termed CCF-1 or coelomic cytolytic factor 1) can be adsorbed on the surface of opsonized particles and may be involved in opsonizing and hemolytic effects of coelomic fluid.
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Antimicrobial defense of the earthworm
