Localization of basic and acidic argyrophilic proteins in nuclear structures of elongating mouse spermatids
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
7873803
Knihovny.cz E-resources
- MeSH
- Alkalies MeSH
- Silver Staining MeSH
- Nuclear Proteins analysis MeSH
- Acids MeSH
- Mice MeSH
- Spermatids chemistry ultrastructure MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Alkalies MeSH
- Nuclear Proteins MeSH
- Acids MeSH
Different cytochemical techniques at the electron microscopic level were used to investigate the presence of basic and acidic proteins during spermiogenesis in mice. Basic lysine rich proteins were demonstrated with an ethanolic solution of phosphotungstic acid, acidic agryrophilic proteins using two silver staining procedures applied en bloc and on the ultrathin lowicryl sections. Both types of proteins had very similar localization in the nucleoplasm and identical distribution within the postacrosomal dense lamina on the surface of late spermatid heads. The specificity of reactions is briefly discussed.
