Capillary gas chromatography of underivatized fatty acids with a free fatty acid phase column and a programmed temperature vaporizer injector
Language English Country Netherlands Media print
Document type Journal Article
- MeSH
- Food Analysis MeSH
- Chromatography, Gas MeSH
- Phosphatidylcholines blood MeSH
- Fatty Acids, Nonesterified chemistry MeSH
- Humans MeSH
- Fatty Acids analysis blood chemistry MeSH
- Temperature MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Phosphatidylcholines MeSH
- Fatty Acids, Nonesterified MeSH
- Fatty Acids MeSH
Underivatized fatty acids occurring in lipids of a number of biological specimens (blood plasma, tissues, food) were studied using a capillary column with a chemically bonded free fatty acid phase and a programmed temperature vaporizer. Non-linear calibration dependences were obtained for almost all the acids at lower concentrations because of losses in the column, non-linearity increasing with increasing carbon number and degree of unsaturation. Sample introduction at temperatures below the solvent boiling point eliminates losses in the injector. The lower recovery of long-chain polyunsaturated acids is caused by interactions occurring in the column. Some critical pairs remain unresolved in the underivatized form (18:1n9-18:1n7, 20:4n6-20:3n3), whereas the corresponding methyl esters exhibit baseline resolution.
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