Fast-protein liquid chromatography system as a tool for liposome preparation by the extrusion procedure
Language English Country United States Media print
Document type Journal Article
PubMed
8074292
DOI
10.1006/abio.1994.1191
PII: S0003-2697(84)71191-2
Knihovny.cz E-resources
- MeSH
- Chromatography, Liquid methods MeSH
- Chemistry, Pharmaceutical methods MeSH
- Filtration methods MeSH
- Phospholipids chemical synthesis chemistry MeSH
- Lipid Bilayers chemistry MeSH
- Liposomes chemical synthesis chemistry MeSH
- Proteins MeSH
- Pressure MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Phospholipids MeSH
- Lipid Bilayers MeSH
- Liposomes MeSH
- Proteins MeSH
Employment of fast-protein liquid chromatography (FPLC) system and a high-pressure filtration cell is described for the production of liposomes by the rapid extrusion technique. The system allows processing of up to 50 ml liposomal suspension in one batch at a defined temperature and applied pressure of up to 4.5 MPa. This relatively simple technique is applicable in any laboratory equipped with FPLC or HPLC systems.
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