Acid phosphatase synthesis in Aspergillus flavus
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
8549995
DOI
10.1007/bf02814065
Knihovny.cz E-resources
- MeSH
- Aspergillus flavus enzymology growth & development MeSH
- Glucose metabolism MeSH
- Magnesium pharmacology MeSH
- Enzyme Inhibitors pharmacology MeSH
- Kinetics MeSH
- Hydrogen-Ion Concentration MeSH
- Acid Phosphatase antagonists & inhibitors biosynthesis chemistry isolation & purification metabolism MeSH
- Molecular Weight MeSH
- Ammonium Sulfate metabolism MeSH
- Temperature MeSH
- Calcium pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Glucose MeSH
- Magnesium MeSH
- Enzyme Inhibitors MeSH
- Acid Phosphatase MeSH
- Ammonium Sulfate MeSH
- Calcium MeSH
Proteins with phosphatase activity were produced during the growth of Aspergillus flavus in a phosphate-supplemented liquid synthetic medium. The best carbon and nitrogen sources for the synthesis of phosphatase were glucose and ammonium sulfate, respectively. The proteins were separated by molecular exclusion and ion exclusion chromatography (IEC) into three components one of which showed phosphatase activity. The molar mass of the enzyme was approximately 62 kDa. The purified enzyme exhibited an optimum activity at pH 4.0 and at 45 degrees C. The activity of the enzyme was stimulated by Ca2+ and Mg2+ but inhibited by fluoride, iodoacetic acid, ethylenediaminetetraacetic acid and 2,4-dinitrophenol, and exhibited an apparent KM of approximately 420 mumol/L.
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Appl Environ Microbiol. 1991 Sep;57(9):2447-52 PubMed
Biochemistry. 1977 Sep 6;16(18):4020-5 PubMed
FEMS Microbiol Lett. 1990 Sep 1;59(1-2):111-5 PubMed
Biochem J. 1972 Mar;127(1):87-96 PubMed
Biochim Biophys Acta. 1972 May 12;268(2):431-41 PubMed
Eur J Biochem. 1973 Mar 15;33(3):584-92 PubMed
J Bacteriol. 1978 Oct;136(1):19-23 PubMed
J Gen Microbiol. 1971 Mar;65(3):291-303 PubMed
Annu Rev Microbiol. 1976;30:41-62 PubMed
Biochim Biophys Acta. 1960 Mar 11;38:460-9 PubMed
Nucleic Acids Res. 1984 Oct 25;12(20):7721-39 PubMed
Microbiol Rev. 1979 Sep;43(3):361-83 PubMed
Arch Mikrobiol. 1974 Apr 10;97(1):63-7 PubMed
