Mutagenicity of cytostatic drugs in a bacterial system. II. DNA-repair test
Language English Country United States Media print
Document type Comparative Study, Journal Article
PubMed
8763145
DOI
10.1007/bf02814723
Knihovny.cz E-resources
- MeSH
- DNA, Bacterial drug effects MeSH
- Escherichia coli drug effects genetics MeSH
- DNA Repair drug effects MeSH
- DNA Damage MeSH
- Antineoplastic Agents pharmacology toxicity MeSH
- Drug Screening Assays, Antitumor MeSH
- Mutagenicity Tests * MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Antineoplastic Agents MeSH
A liquid micromethod modification of the DNA-repair test using an automatic growth analyzer was developed. The wild strain Escherichia coli WP2 and six repair-deficient isogenic test strains were used. To compare this repair test with the conventional plate Ames test, a set of nine cytostatics were tested. Cloturin, adriamycin, mitoxantron, oracin, lomustine and tris(2-chloroethyl)amine were found to be positive, and 6-mercaptopurine, butocin and cyclophosphamide negative. The experimental micromethod appears to be useful for assessing the differential lethality in bacteria and can be combined in any short-term test system to predict genotoxicity.
See more in PubMed
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Mutagenicity of cytostatic drugs in a bacterial system. I. Ames test
