Determination of adenosine phosphates in whole blood by capillary zone electrophoresis
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Adenine Nucleotides blood MeSH
- Adenosine Diphosphate blood MeSH
- Adenosine Monophosphate blood MeSH
- Adenosine Triphosphate blood MeSH
- Electrophoresis, Capillary methods MeSH
- Humans MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenine Nucleotides MeSH
- Adenosine Diphosphate MeSH
- Adenosine Monophosphate MeSH
- Adenosine Triphosphate MeSH
The pool of chemical energy in an organism represented by high-energy compounds can be assessed by means of adenosine triphosphate (ATP) determination in whole blood and tissues. The elegant manner for the determination of adenosine phosphates (ATP, ADP, AMP) in a single assay is offered by the technique of capillary zone electrophoresis. For this purpose, the BioFocus 3000 Capillary Electrophoresis System (BIO-RAD Laboratories, Inc., Hercules, CA, USA) was used. For the construction of calibration curves, pure preparations of ATP, ADP and AMP were analyzed. The method was used for adenosine phosphates determination in the umbilical blood samples from physiological and immature newborns. Capillary zone electrophoresis enables a specific and simultaneous determination of adenosine phosphates and, thus, monitoring of unusual metabolic situations.
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