Application of a colorimetric DNA-DNA hybridization method for identification of Aeromonas genomic species, isolated from diarrheal stools
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
9131804
DOI
10.1007/bf02814668
Knihovny.cz E-resources
- MeSH
- Aeromonas classification genetics isolation & purification MeSH
- Diphenylamine MeSH
- Child MeSH
- DNA, Bacterial analysis genetics MeSH
- Adult MeSH
- Species Specificity MeSH
- Feces microbiology MeSH
- Gram-Negative Bacterial Infections microbiology MeSH
- Nucleic Acid Hybridization methods MeSH
- Indicators and Reagents MeSH
- Infant MeSH
- Colorimetry methods MeSH
- Humans MeSH
- Child, Preschool MeSH
- Diarrhea microbiology MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Humans MeSH
- Child, Preschool MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Diphenylamine MeSH
- DNA, Bacterial MeSH
- Indicators and Reagents MeSH
The colorimetric DNA-DNA hybridization method for the identification of 18 strains of Aeromonas spp. isolated from human stools was used. Bacterial isolates were also examined by phenotypic characteristics. On the basis of biochemical tests 13 strains were included in phenogroup A. caviae and 5 strains in A. sobria. Identification to the species level was obtained by colorimetric hybridization method. DNA-DNA similarity values showed that isolates of A. caviae group belong to hybridization group (HG) 4 whereas isolates of A. sobria belong to HG 8/10. DNA relatedness results obtained by the colorimetric method showed good agreement with values detected by the spectrophotometric method. The background in the colorimetric method is lower than in the spectrophotometric one. Results of this study indicate the usefulness of the colorimetric DNA-DNA hybridization in microplates method for the identification of Aeromonas genomic species, isolated from human diarrheal stools.
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