For parallel detection of mycobacteria by the radiometric method using a Bactec 460 TB apparatus and by the standard cultivation method 5383 specimens of pathological material from patients of in-patient TB departments (90%) and other health facilities (10%) were examined. 492 samples were positive. By the radiometric method alone 114 samples were positive and by cultivation only 15 samples. The mean period of detection was 12.9 days for the Bactec method, for the cultivation method 32.4 days. For the Bactec system 0.4 ml inoculum was used and one cultivation vessel, for the cultivation 0.4-0.6 ml inoculum and two egg media per sample. Of 15 samples positive on cultivation only, rarely colonies were obtained after 6-9 weeks' incubation.

Oddĕlení mikrobiologie Fakultní nemocnice Plzen