Rapid screening of destruxins by liquid chromatography/mass spectrometry
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9538526
DOI
10.1002/(sici)1096-9888(199803)33:3<274::aid-jms630>3.0.co;2-r
PII: 10.1002/(SICI)1096-9888(199803)33:3<274::AID-JMS630>3.0.CO;2-R
Knihovny.cz E-resources
- MeSH
- Chromatography, Liquid MeSH
- Peptides, Cyclic analysis MeSH
- Mass Spectrometry MeSH
- Mitosporic Fungi chemistry MeSH
- Spectrophotometry, Ultraviolet MeSH
- Spectrometry, Mass, Fast Atom Bombardment MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Peptides, Cyclic MeSH
A liquid chromatographic/mass spectrometric (LC/MS) method utilizing an atmospheric pressure chemical ionization (APCI) interface and in-source collisionally induced dissociation (CID) was developed for the rapid screening of the cyclic hexadepsipeptides destruxins, produced by the fungi Metarhizium anisopliae and Trichothecium roseum. The APCI mass spectra are fully consistent with the high-energy CID data but the sequence ions are abundant over the whole mass range. In addition to 22 known destruxins, two new representatives of this family were detected by LC/MS analysis: destruxin Ed1 and roseotoxin A were isolated and their structures were inferred from MS/MS and NMR data.
References provided by Crossref.org
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