Characterization of ftsZ gene and its protein product from Streptomyces collinus producing kirromycin
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9712736
DOI
10.1006/bbrc.1998.9193
PII: S0006291X98991938
Knihovny.cz E-resources
- MeSH
- Bacterial Proteins chemistry genetics metabolism MeSH
- Cytoskeletal Proteins * MeSH
- Escherichia coli chemistry MeSH
- Guanosine Triphosphate metabolism MeSH
- Molecular Sequence Data MeSH
- Molecular Weight MeSH
- Peptide Fragments chemistry MeSH
- Restriction Mapping MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Analysis MeSH
- Sequence Homology MeSH
- Sequence Alignment MeSH
- Blotting, Southern MeSH
- Streptomyces chemistry genetics growth & development MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Cytoskeletal Proteins * MeSH
- FtsZ protein, Bacteria MeSH Browser
- Guanosine Triphosphate MeSH
- Peptide Fragments MeSH
The FtsZ protein is required for septation and conversion of aerial mycelium into chains of streptomycete spores. We have cloned and sequenced the ftsZ gene from Streptomyces collinus. The ftsZ of S. collinus is not in juxtaposition with ftsA and IpxC as in Escherichia coli or Bacillus subtilis. The gene encodes a polypeptide of 402 amino acid residues with a molecular mass of 41.3 kDa. N-terminus shares a high level of sequence similarity with FtsZ of S. coelicolor and S. griseus, respectively. C-terminal part is variable both in length and sequence. The purified protein binds GTP. Using polyclonal antisera against FtsZ, we have found that the protein is expressed at the beginning of germination of spores and is present in vegetative cells and aerial mycelium, but not in spores.
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General and molecular microbiology and microbial genetics in the IM CAS
GENBANK
AF073487
