Electrochemical biosensors for DNA hybridization and DNA damage
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9828356
DOI
10.1016/s0956-5663(98)00017-7
PII: S0956-5663(98)00017-7
Knihovny.cz E-resources
- MeSH
- Biosensing Techniques * MeSH
- DNA analysis genetics MeSH
- Nucleic Acid Hybridization * MeSH
- Humans MeSH
- DNA Damage * MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA MeSH
Recent trends in the development of DNA biosensors for nucleotide sequence-specific DNA hybridization and for the detection of the DNA damage are briefly reviewed. Changes in the redox signals of base residues in DNA immobilized at the surface of carbon or mercury electrodes can be used as a sign of the damage of DNA bases. Some compounds interacting with DNA can produce their own redox signals on binding to DNA. Covalently closed circular (usually supercoiled) DNA attached to the electrode surface can be used for a sensitive detection of a single break of the DNA sugar-phosphate backbone and for detection of agents cleaving the DNA backbone such as hydroxyl radicals, ionizing radiation, nucleases, etc. Using the peptide nucleic acid in the biosensor recognition layer greatly increased the specificity of the DNA hybridization biosensor making it possible to detect point mutations (single-base mismatches) in DNA.
References provided by Crossref.org
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