Atypical location of double-strand origin of replication (nic site) on the plasmid pGA1 from Corynebacterium glutamicum
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12422507
DOI
10.1007/bf02818687
Knihovny.cz E-resources
- MeSH
- Corynebacterium genetics MeSH
- DNA, Bacterial genetics MeSH
- Chromosome Mapping MeSH
- Plasmids genetics MeSH
- Replication Origin * MeSH
- Base Sequence MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
The double-strand origin of replication (dso) of the rolling-circle-replicating (RC) plasmid pGA1 from Corynebacterium glutamicum was analyzed using the runoff DNA synthesis assay. The site- and strand-specific breakage of double-stranded plasmid DNA, representing the nic site of dso, was localized precisely within the sequence 5'-CTGG decreases AT-3' in the distal part of the pGA1 rep gene. This location of dso differs from the dso positions found on other RC plasmids and is in agreement with the classification of the plasmid pGA1 into a new group of RC plasmids.
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Control of rep gene expression in plasmid pGA1 from Corynebacterium glutamicum
