LR White is preferable to Unicryl for immunogold detection of fixation-sensitive nuclear antigens
Language English Country Italy Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
12597621
DOI
10.4081/1748
Knihovny.cz E-resources
- MeSH
- Acrylic Resins * MeSH
- Antigens, Nuclear chemistry isolation & purification MeSH
- Tissue Fixation methods MeSH
- HeLa Cells MeSH
- Microscopy, Immunoelectron MeSH
- Immunohistochemistry * MeSH
- Indicators and Reagents MeSH
- Humans MeSH
- Ultraviolet Rays MeSH
- Tissue Embedding MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Acrylic Resins * MeSH
- Antigens, Nuclear MeSH
- Indicators and Reagents MeSH
- LR white MeSH Browser
- unicryl MeSH Browser
The purpose of this study was to compare two electron microscopy embedding media - LR White and Unicryl - with regard to cell morphologyical and immunohistochemical preservation properties for the study of fixation-sensitive nuclear antigens. Human cervical carcinoma (HeLa) cells were fixed with 2% paraformaldehyde and 0.1% glutaraldehyde, and embedded in parallel in the two resins: LR White and Unicryl using; two different polymerization protocols were used for each resin. Preservation of fine nuclear structure was good after LR White and poor after Unicryl embedding. Immunogold labeling of Sm antigen was significantly stronger on LR White sections. Polymerization by UV light resulted in stronger and more specific labeling than heat polymerization. These results show that LR White is advantageous over Unicryl for the study of nuclear antigens requiring delicate aldehyde fixation.
References provided by Crossref.org
