Expression of macrophage inflammatory protein-3 beta/CCL19 in pulmonary sarcoidosis
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12626344
DOI
10.1164/rccm.200205-487oc
PII: 200205-487OC
Knihovny.cz E-resources
- MeSH
- Lymphocyte Activation drug effects immunology MeSH
- Macrophages, Alveolar drug effects immunology MeSH
- Bronchoalveolar Lavage Fluid chemistry MeSH
- Chemokine CCL19 MeSH
- Chemokine CCL20 MeSH
- Chemokines, CC analysis genetics immunology MeSH
- Cyclosporine immunology therapeutic use MeSH
- Dexamethasone immunology therapeutic use MeSH
- Adult MeSH
- Down-Regulation drug effects MeSH
- Gene Expression * drug effects genetics immunology MeSH
- Immunohistochemistry MeSH
- Middle Aged MeSH
- Humans MeSH
- Macrophage Inflammatory Proteins analysis genetics immunology MeSH
- RNA, Messenger analysis MeSH
- Sarcoidosis, Pulmonary blood drug therapy immunology pathology MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Disease Progression MeSH
- Receptors, CCR6 MeSH
- Receptors, Chemokine * MeSH
- Aged MeSH
- Case-Control Studies MeSH
- Severity of Illness Index MeSH
- T-Lymphocytes drug effects immunology MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CCL19 protein, human MeSH Browser
- CCL20 protein, human MeSH Browser
- CCR6 protein, human MeSH Browser
- Chemokine CCL19 MeSH
- Chemokine CCL20 MeSH
- Chemokines, CC MeSH
- Cyclosporine MeSH
- Dexamethasone MeSH
- Macrophage Inflammatory Proteins MeSH
- RNA, Messenger MeSH
- Receptors, CCR6 MeSH
- Receptors, Chemokine * MeSH
In this study, messenger RNA (mRNA) expression for novel T lymphocyte chemoattractants, leukotactin-1, macrophage inflammatory protein (MIP)-3 alpha and MIP-3 beta was investigated in bronchoalveolar lavage fluid (BALF) cells from patients with sarcoidosis, a T cell-mediated disease with typical CD4+ lymphocyte alveolitis. Of these three chemokines, only MIP-3 beta mRNA was upregulated in sarcoidosis, and therefore, protein levels of this chemokine, its pharmacologic regulation, and association with disease clinical course were explored. MIP-3 beta protein concentrations were elevated in BALF from sarcoid patients compared with control subjects (p = 0.001) and in patients with chest X-ray stage II chemokine protein levels were increased compared with stage I (p = 0.003). MIP-3 beta protein was associated predominantly with alveolar macrophages and correlated with BALF lymphocytes and T cell subsets. mRNA expression for the MIP-3 beta receptor, CC chemokine receptor 7, was increased in sarcoidosis and correlated with MIP-3 beta protein levels. MIP-3 beta mRNA and protein expression in BALF cells was suppressed by dexamethasone and cyclosporine A in vitro. In conclusion, MIP-3 beta is implicated in T lymphocyte recruitment in sarcoidosis, is associated with disease progression, and is downregulated by drugs used for sarcoidosis treatment. This novel chemokine, therefore, represents a candidate for studies of sarcoidosis pathobiologic mechanisms.
References provided by Crossref.org
In vitro pharmacoregulation of CC chemokine ligand 5 and its receptor CCR5 in diffuse lung diseases
