Main flavonoids in the root of Scutellaria baicalensis cultivated in Europe and their comparative antiradical properties
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12820232
DOI
10.1002/ptr.1216
Knihovny.cz E-zdroje
- MeSH
- bifenylové sloučeniny MeSH
- flavanony * MeSH
- flavonoidy aplikace a dávkování chemie farmakologie terapeutické užití MeSH
- fytoterapie * MeSH
- glukuronidy chemie MeSH
- hydroxylový radikál MeSH
- inhibiční koncentrace 50 MeSH
- kořeny rostlin MeSH
- lidé MeSH
- pikráty MeSH
- rostlinné extrakty aplikace a dávkování farmakologie terapeutické užití MeSH
- scavengery volných radikálů aplikace a dávkování farmakologie terapeutické užití MeSH
- šišák bajkalský * MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Evropa MeSH
- Názvy látek
- 1,1-diphenyl-2-picrylhydrazyl MeSH Prohlížeč
- baicalin MeSH Prohlížeč
- bifenylové sloučeniny MeSH
- flavanony * MeSH
- flavonoidy MeSH
- glukuronidy MeSH
- hydroxylový radikál MeSH
- pikráty MeSH
- rostlinné extrakty MeSH
- scavengery volných radikálů MeSH
- wogonin-7-glucuronide MeSH Prohlížeč
The content of the main flavonoids in the root of Scutellaria baicalensis Georgi cultivated in Central Europe was evaluated using the new simple RP-HPLC method with gradient of acetonitrile in mobile phase. The main components of the roots were baicalin (8.12% of dry root mass) and wogonin glucuronide (2.52%). The content of flavonoids was comparable with the content in plants cultivated in natural localities. Five main flavonoids were evaluated for their scavenging ability with DPPH radical-generating system and due to limited solubility only two flavonoids were investigated for their ability to scavenge hydroxyl radical by the aromatic hydroxylation method. The total extract was also tested in both the experimental arrangements. In experiments with DPPH, only baicalin and baicalein displayed a significant scavenging effect, while the production of OH radicals generated by UV photolysis of H(2)O(2) was considerable decreased in the presence of baicalin and wogonin glucuronide. After comparison with results obtained for the total extract, it was concluded, that the scavenging activity of the extract against DPPH is mainly derived from baicalin. On the other hand, baicalin, wogonin glucuronide and probably other flavonoids participate in scavenging OH radical.
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