Continuous penicillin G hydrolysis in an electro-membrane reactor with immobilized penicillin G acylase
Biotechnology letters.
2003 Mar ;
25 (6) :
485-90.
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/pmid12882276
- MeSH
- Enzyme Activation MeSH
- Bioreactors * MeSH
- Equipment Design MeSH
- Electrochemistry instrumentation methods MeSH
- Electromagnetic Fields MeSH
- Enzymes, Immobilized chemistry MeSH
- Hydrolysis MeSH
- Penicillanic Acid analogs & derivatives chemical synthesis MeSH
- Membranes, Artificial * MeSH
- Penicillin G chemistry MeSH
- Penicillin Amidase chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- aminopenicillanic acid MeSH Browser
- Enzymes, Immobilized MeSH
- Penicillanic Acid MeSH
- Membranes, Artificial * MeSH
- Penicillin G MeSH
- Penicillin Amidase MeSH
Penicillin G (2%, w/v in phosphate buffer, pH 8) was hydrolysed in a flow-through, miniature electro-membrane reactor with the penicillin G acylase immobilized in 5% (w/v) polyacrylamide (diam. 10 mm, thickness 2.6 mm, enzyme activity 24 U ml(-1)). The conversion of penicillin G increased from 0.15 to almost 0.5 when the electric current applied to the reactor was changed from -600 to +600 A/m2 with a substrate residency of 1 h.
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