Targeted disruption of Streptomyces globisporus lndF and lndL cyclase genes involved in landomycin E biosynthesis
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
14533479
DOI
10.1007/bf02931329
Knihovny.cz E-resources
- MeSH
- Aminoglycosides biosynthesis chemistry MeSH
- Genes, Bacterial physiology MeSH
- Bacterial Proteins genetics MeSH
- Chromatography, Thin Layer MeSH
- Enzymes genetics metabolism MeSH
- Mutagenesis, Insertional * MeSH
- Blotting, Southern MeSH
- Streptomyces chemistry enzymology genetics MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Aminoglycosides MeSH
- Bacterial Proteins MeSH
- Enzymes MeSH
- landomycin E MeSH Browser
Streptomyces globisporus strains with knockouts in lndF and lndL genes, previously identified as possibly encoding cyclases governing cyclization of the nascent oligoketide ('polyketide') chain during the biosynthesis of the antitumor angucycline landomycin E, were prepared. On combining the results of sequence analysis and HPLC of extracts from mutant strains, lndL was suggested to control the first cyclization-aromatization event and lndF to be responsible for the 3rd-4th ring formation.
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