Rice Na+/H+-antiporter Nhx1 partially complements the alkali-metal-cation sensitivity of yeast strains lacking three sodium transporters
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15702539
DOI
10.1007/bf02931527
Knihovny.cz E-resources
- MeSH
- DNA, Plant genetics MeSH
- Genes, Fungal MeSH
- Metals metabolism pharmacology MeSH
- Mutation MeSH
- Sodium-Hydrogen Exchangers genetics metabolism MeSH
- Recombinant Proteins genetics metabolism MeSH
- Genes, Plant MeSH
- Oryza genetics metabolism MeSH
- Saccharomyces cerevisiae Proteins genetics metabolism MeSH
- Saccharomyces cerevisiae drug effects genetics metabolism MeSH
- Base Sequence MeSH
- Genetic Complementation Test MeSH
- Vacuoles metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Plant MeSH
- Metals MeSH
- Sodium-Hydrogen Exchangers MeSH
- Recombinant Proteins MeSH
- Saccharomyces cerevisiae Proteins MeSH
A triple mutant strain of Saccharomyces cerevisiae lacking its own Na+-ATPases and Na+/H+ antiporters (enal-4delta nha1delta nhx1delta) was used for the expression of the Oryza sativa NHX1 gene encoding a putative vacuolar Na+/H+ exchanger. Upon expression in yeast cells, the OsNhx 1p is not a transport system specific only for sodium cations but it has a broad substrate specificity for at least four alkali metal cations (Na+, Li+, K+ and Rb+) and is able to substitute for the endogenous yeast ScNhx1 antiporter. Its activity contributes to sequestration of alkali metal cations in intracellular vesicles.
See more in PubMed
FEMS Microbiol Lett. 1999 Feb 15;171(2):167-72 PubMed
Trends Plant Sci. 2003 May;8(5):200-1 PubMed
Yeast. 1986 Sep;2(3):163-7 PubMed
Cell. 1989 Jul 28;58(2):409-19 PubMed
Microbiology. 1999 Nov;145 ( Pt 11):3221-8 PubMed
Plant Physiol. 1995 Nov;109(3):735-742 PubMed
Mol Cell Biol. 1987 Nov;7(11):4082-8 PubMed
Mol Gen Genet. 1969;105(4):314-6 PubMed
Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):9061-6 PubMed
J Exp Bot. 2002 May;53(371):1055-65 PubMed
Mol Microbiol. 2001 May;40(3):656-68 PubMed
Mol Biol Cell. 2000 Dec;11(12):4277-94 PubMed
Nature. 2003 Oct 16;425(6959):686-91 PubMed
Microbiology. 2002 Apr;148(Pt 4):1225-32 PubMed
J Biol Chem. 1998 Jun 12;273(24):14838-44 PubMed
J Biol Chem. 1989 May 5;264(13):7437-46 PubMed
J Biol Chem. 1998 Aug 14;273(33):21054-60 PubMed
Plant J. 2002 Jun;30(5):529-39 PubMed
Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1480-5 PubMed
Biochim Biophys Acta. 2000 Mar 10;1469(1):1-30 PubMed
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6896-901 PubMed
J Biol Chem. 2001 Aug 10;276(32):29740-7 PubMed
EMBO J. 1995 Aug 15;14(16):3870-82 PubMed
J Biol Chem. 2002 Jan 25;277(4):2413-8 PubMed
FEBS Lett. 1996 May 27;387(1):89-93 PubMed
Biochem J. 2000 Oct 1;351(Pt 1):241-9 PubMed
Science. 1999 Aug 20;285(5431):1256-8 PubMed
Plant Cell Physiol. 2004 Feb;45(2):146-59 PubMed
J Biol Chem. 1998 Jan 16;273(3):1640-6 PubMed
J Biol Chem. 1997 Oct 17;272(42):26145-52 PubMed
Microbiology. 1998 Oct;144 ( Pt 10):2749-58 PubMed
Curr Opin Cell Biol. 2000 Aug;12(4):431-4 PubMed
FEBS Lett. 1991 Oct 21;291(2):189-91 PubMed
Biochim Biophys Acta. 1999 Jul 7;1446(1-2):149-55 PubMed
FEBS Lett. 2000 Apr 14;471(2-3):224-8 PubMed
Physiol Plant. 2002 Oct;116(2):206-212 PubMed
Ann Bot. 2003 Apr;91(5):503-27 PubMed
Exploration of yeast alkali metal cation/H+ antiporters: sequence and structure comparison
