DNA isolation from museum and type collection slides of microsporidia
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
15955346
DOI
10.1016/j.jip.2005.02.004
PII: S0022-2011(05)00047-9
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- DNA Primers MeSH
- DNA MeSH
- Microsporidia genetics MeSH
- Museums MeSH
- Specimen Handling MeSH
- Polymerase Chain Reaction MeSH
- DNA, Protozoan isolation & purification MeSH
- Tissue Preservation MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA Primers MeSH
- DNA MeSH
- DNA, Protozoan MeSH
DNA from 19 species of microsporidia was isolated and amplified from infected host tissue that were originally prepared between the years 1946 and 1996. The smears, on glass microscope slides, were either Giemsa-stained or unstained. Methanol-fixed, Giemsa-stained smears proved to be suitable for DNA isolation; DNA was amplified from only two of 14 unstained slides. The isolated DNA was successfully amplified in PCRs using small subunit and large subunit rDNA primers and sequenced. The high efficiency of DNA isolation demonstrates the usefulness of archival and type collection slides for some molecular biology and molecular taxonomy studies.
References provided by Crossref.org
