Development of cytochrome-c oxidase activity in rat heart: downregulation in newborn rats
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16043886
DOI
10.1385/cbb:43:1:087
PII: CBB:43:1:087
Knihovny.cz E-resources
- MeSH
- Enzyme Activation drug effects MeSH
- Detergents administration & dosage MeSH
- Down-Regulation drug effects MeSH
- Rats MeSH
- Cells, Cultured MeSH
- Myocardium enzymology MeSH
- Animals, Newborn MeSH
- Rats, Wistar MeSH
- Gene Expression Regulation, Enzymologic drug effects physiology MeSH
- Electron Transport Complex IV metabolism MeSH
- Heart drug effects MeSH
- Mitochondria, Heart drug effects enzymology MeSH
- Aging drug effects metabolism MeSH
- Gene Expression Regulation, Developmental drug effects physiology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Detergents MeSH
- Electron Transport Complex IV MeSH
Cytochrome-c oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period.
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