HPLC separácia racemátov bazických esterov alkoxyfenylkarbámovej kyseliny pouzitím dvoch teikoplanínových chirálnych stacionárnych fáz
[HPLC separation of racemic basic esters of alkoxyphenylcarbamic acid using two teicoplanin chiral stationary phases]
Language Czech Country Czech Republic Media print
Document type English Abstract, Journal Article, Research Support, Non-U.S. Gov't
PubMed
16124198
- MeSH
- Anti-Bacterial Agents chemistry MeSH
- Carbamates analysis MeSH
- Stereoisomerism MeSH
- Teicoplanin analogs & derivatives chemistry MeSH
- Chromatography, High Pressure Liquid * MeSH
- Publication type
- English Abstract MeSH
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Carbamates MeSH
- phenylcarbamic acid MeSH Browser
- teicoplanin aglycone MeSH Browser
- Teicoplanin MeSH
This paper presents the results obtained with the use of two chiral stationary phases (CSP), based on a glycopeptide antibiotic agent--teicoplanin aglycone (CHIROBIOTIC TAG) and methylated teicoplanin aglycon m-TAG. Twenty-one racemic mixtures of 1-methyl-2-piperidinoethylesters of 2-, 3- and 4-alkoxyphenylcarbamic acid were examined. The investigation included interaction between separated substances and CSP, and the effect of separation of the enantiomers under study on the value of the resolution factor (R(ij)) under identical chromatographic conditions with the use of the method of high-performance liquid chromatography (HPLC). On the basis of obtained results, it is possible to report that CSP-CHIROBIOTIC TAG is more advantageous for these racemates, because it does not contain a saccharide part, with decrease the possibility of non-polar interactions which exert a negative effect on the R(ij) value.
