Paenibacillus mendelii sp. nov., from surface-sterilized seeds of Pisum sativum L
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16280495
DOI
10.1099/ijs.0.63759-0
Knihovny.cz E-resources
- MeSH
- DNA, Bacterial analysis chemistry isolation & purification MeSH
- Phylogeny MeSH
- Genes, rRNA MeSH
- Gram-Positive Bacteria classification isolation & purification physiology MeSH
- Pisum sativum microbiology MeSH
- Nucleic Acid Hybridization MeSH
- Fatty Acids analysis isolation & purification MeSH
- Molecular Sequence Data MeSH
- DNA, Ribosomal chemistry isolation & purification MeSH
- RNA, Ribosomal, 16S analysis genetics MeSH
- Sequence Analysis, DNA MeSH
- Seeds microbiology MeSH
- Base Composition MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Fatty Acids MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
A Gram-variable, facultatively anaerobic, endospore-forming bacterium was isolated from surface-sterilized seeds of the garden pea and characterized with phenotypic and molecular methods. A PCR with the Paenibacillus-specific primer PAEN515F and the 16S rRNA gene sequence indicated that strain C/2T belongs to the genus Paenibacillus and is closely related to Paenibacillus phyllosphaerae (94.0 % sequence similarity). Strain C/2T generated a unique phenotypic profile, in particular for the production of acid from substrates. The DNA G+C content (50.8 mol%) and the major fatty acid (anteiso-C(15 : 0)) are consistent with the genus Paenibacillus. DNA-DNA hybridization distinguished strain C/2T from other phylogenetically related Paenibacillus species and, therefore, strain C/2T (=CCM 4839T=LMG 23002T) is here described as the type strain of a novel species, for which the name Paenibacillus mendelii sp. nov. is proposed.
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GENBANK
AF537343
