Dynamics of PCR-based diagnosis in patients with invasive meningococcal disease
Language English Country Great Britain, England Media print
Document type Comparative Study, Journal Article
PubMed
16441451
DOI
10.1111/j.1469-0691.2005.01327.x
PII: S1198-743X(14)63397-8
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- Child MeSH
- DNA, Bacterial analysis genetics MeSH
- Adult MeSH
- Infant MeSH
- Blood microbiology MeSH
- Middle Aged MeSH
- Humans MeSH
- Meningitis, Meningococcal diagnosis MeSH
- Meningococcal Infections diagnosis MeSH
- Adolescent MeSH
- Cerebrospinal Fluid microbiology MeSH
- Neisseria meningitidis genetics growth & development isolation & purification MeSH
- Polymerase Chain Reaction methods MeSH
- Child, Preschool MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Infant MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- DNA, Bacterial MeSH
Invasive meningococcal disease continues to be a life-threatening condition and rapid diagnosis is important for the administration of appropriate treatment. This study focused on the use of PCR for the diagnosis of meningococcal aetiology and the dynamics of PCR-based diagnosis over time in various biological samples. Sixty cerebrospinal fluid (CSF) and 144 serum samples collected during the first week of hospitalisation from 37 patients with laboratory-confirmed invasive meningococcal disease were investigated. Overall, 91.9% of CSF samples and 45.9% of serum samples were PCR-positive, while culture of CSF and blood was positive for only 35% and 39% samples, respectively. Positive PCR results were obtained until day 7 with CSF and until day 5 with serum. It is therefore recommended that samples for molecular diagnosis should be collected early in the course of suspected invasive meningococcal disease.
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