Explantátová kultura Trifolium pratense L
[Explant culture of Trifolium pratense L]
Language Czech Country Czech Republic Media print
Document type English Abstract, Journal Article
PubMed
16502812
- MeSH
- Flavonoids biosynthesis MeSH
- Culture Techniques MeSH
- Trifolium metabolism MeSH
- Publication type
- English Abstract MeSH
- Journal Article MeSH
- Names of Substances
- Flavonoids MeSH
The red cloverl seems to be a prospective source of flavonoids and isoflavonoids with phytoestrogenic activity. Young seedlings of four different varieties of Trifolium pratense L. were employed to derive callus and suspension cultures. The optimal medium for cultivation is Gamborg's cultivating medium with an addition of 2 mg x l(-1) of 2,4-dichlorphenoxyacetic acid and 2 mg x l(-1) of 6-benzylaminopurine. On the basis of growth and production characteristics, subcultivating intervals of 29 to 43 days in callus culture and 16 to 23 days in suspension culture were determined. TLC and HPLC revealed that the explant culture of Trifolium pratense L. contains the isoflavonoid formononetin.