Surface plasmon resonance biosensor for direct detection of antibody against Epstein-Barr virus
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16797175
DOI
10.1016/j.bios.2006.04.021
PII: S0956-5663(06)00226-0
Knihovny.cz E-resources
- MeSH
- Equipment Failure Analysis MeSH
- Biosensing Techniques instrumentation methods MeSH
- Equipment Design MeSH
- Immunoassay instrumentation methods MeSH
- Antibodies analysis immunology MeSH
- Epstein-Barr Virus Nuclear Antigens analysis immunology MeSH
- Herpesvirus 4, Human immunology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- EBV-encoded nuclear antigen 1 MeSH Browser
- Antibodies MeSH
- Epstein-Barr Virus Nuclear Antigens MeSH
This paper describes the direct label-free detection of antibodies against the Epstein-Barr virus (anti-EBNA) using a surface plasmon resonance (SPR) biosensor. The antibody detection was performed using the immunoreaction between anti-EBNA and a respective synthetic peptide (EBNA-1), which was conjugated with bovine serum albumin (BSA-EBNA) and immobilized on the sensor surface. Three immobilization chemistries for the attachment of BSA-EBNA were investigated to optimize ligand density and minimize loss of EBNA-1 immunoreactivity. The developed SPR biosensor functionalized with the optimal immobilization method was calibrated and characterized in terms of detection limit, reproducibility, regenerability and storability. It was demonstrated that the sensor is capable of detecting concentrations of anti-EBNA as low as 0.2 ng/ml (approximately 1 pM) both in buffer and 1% human serum and can be stored and regenerated for repeated use.
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