Mild hydrolysis of nitriles by Fusarium solani strain O1
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17007419
DOI
10.1007/bf02931806
Knihovny.cz E-resources
- MeSH
- Aminohydrolases biosynthesis MeSH
- Fusarium enzymology growth & development MeSH
- Hydrolysis MeSH
- Cells, Immobilized metabolism microbiology MeSH
- Nitriles metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Aminohydrolases MeSH
- nitrilase MeSH Browser
- Nitriles MeSH
High levels of an aromatic nitrilase (about 37 microkat/L culture) were induced in Fusarium solani O1 after transfer of the mycelium from a rich medium into a medium with 20 mmol/L picolinonitrile. The mycelium was entrapped in lense-shaped particles consisting of a polyvinyl alcohol/polyethylene glycol copolymer (LentiKats). The cell-free extract was immobilized by hydrophobic binding onto a Butyl Sepharose column. The enzyme was useful for the mild hydrolysis of nicotinonitrile, isonicotinonitrile and benzonitrile.
See more in PubMed
Appl Environ Microbiol. 2000 Jun;66(6):2290-6 PubMed
Microbiology. 2005 Nov;151(Pt 11):3639-48 PubMed
Arch Biochem Biophys. 1964 Apr;105:133-41 PubMed
Biotechnol Bioeng. 2004 Jun 5;86(5):558-62 PubMed
Biotechnol Bioeng. 2004 May 5;86(3):273-6 PubMed
Anal Biochem. 1976 May 7;72:248-54 PubMed
J Ind Microbiol Biotechnol. 2006 Nov;33(11):891-6 PubMed
Biochem J. 1977 Dec 1;167(3):685-92 PubMed
Biotechnol Appl Biochem. 1992 Jun;15(3):283-302 PubMed
