Comparative proteome analysis of fractions enriched for membrane-associated proteins from Francisella tularensis subsp. tularensis and F. tularensis subsp. holarctica strains
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17081064
DOI
10.1021/pr0601887
Knihovny.cz E-resources
- MeSH
- Electrophoresis, Gel, Two-Dimensional methods MeSH
- Bacterial Proteins chemistry genetics isolation & purification MeSH
- Coloring Agents MeSH
- Enzymes chemistry genetics isolation & purification MeSH
- Francisella tularensis chemistry genetics pathogenicity MeSH
- Francisella chemistry genetics pathogenicity MeSH
- Mass Spectrometry MeSH
- Membrane Proteins chemistry genetics isolation & purification MeSH
- Molecular Sequence Data MeSH
- Peptide Fragments chemistry MeSH
- Proteomics methods MeSH
- Amino Acid Sequence MeSH
- Silver MeSH
- Virulence MeSH
- Computational Biology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Coloring Agents MeSH
- Enzymes MeSH
- Membrane Proteins MeSH
- Peptide Fragments MeSH
- Silver MeSH
The facultative intracellular pathogen Francisella tularensis is the causative agent of the serious infectious disease tularemia. Despite intensive research, the virulence factors and pathogenetic mechanisms remain largely unknown. To identify novel putative virulence factors, we carried out a comparative proteome analysis of fractions enriched for membrane-associated proteins isolated from the highly virulent subspecies tularensis strain SCHU S4 and three representatives of subspecies holarctica of different virulence including the live vaccine strain. We identified six proteins uniquely expressed and four proteins expressed at significantly higher levels by SCHU S4 compared to the ssp. holarctica strains. Four other protein spots represented mass and charge variants and seven spots were charge variants of proteins occurring in the ssp. holarctica strains. The genes encoding proteins of particular interest were examined by sequencing in order to confirm and explain the findings of the proteome analysis. Our studies suggest that the subspecies tularensis-specific proteins represent novel potential virulence factors.
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