Morphological and molecular diversity of the monocercomonadid genera Monocercomonas, Hexamastix, and Honigbergiella gen. nov
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17499022
DOI
10.1016/j.protis.2007.02.003
PII: S1434-4610(07)00017-X
Knihovny.cz E-resources
- MeSH
- Phylogeny MeSH
- Genes, rRNA MeSH
- DNA, Ribosomal Spacer genetics MeSH
- Molecular Sequence Data MeSH
- Organelles ultrastructure MeSH
- DNA, Protozoan chemistry genetics MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Protozoan genetics MeSH
- RNA, Ribosomal, 18S genetics MeSH
- Sequence Analysis, DNA MeSH
- Sequence Homology, Nucleic Acid MeSH
- Microscopy, Electron, Transmission MeSH
- Trichomonadida classification cytology genetics ultrastructure MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Ribosomal Spacer MeSH
- DNA, Protozoan MeSH
- DNA, Ribosomal MeSH
- RNA, Protozoan MeSH
- RNA, Ribosomal, 18S MeSH
The family Monocercomonadidae (Parabasala, Trichomonadida) is characterized by the absence of a costa and in most species also of an undulating membrane; both of which are typical structures of trichomonadids. We have examined 25 isolates of Monocercomonadidae species by sequencing of the SSU rDNA and the ITS region and by light and transmission electron microscopy. The isolates formed three distinct phylogenetically unrelated clades: (1) Monocercomonas colubrorum, (2) Monocercomonas ruminantium together with a strain ATCC 50321 designated as Pseudotrichomonas keilini, and (3) Hexamastix. Twenty isolates of Monocercomonas colubrorum split into three clades with no host-specificity. The morphological differences among clades were insufficient to classify them as a separate species. Non-monophyly of the cattle commensal Monocercomonas ruminantium with the type species Monocercomonas colubrorum and absence of Pseudotrichomonas characters in the free-living strain ATCC 50321 led to their reclassification into a new genus (Honigbergiella gen. nov.). The close relationship of these strains indicates a recent switch between a free-living habit and endobiosis. Two strains of Hexamastix represented different species -Hexamastix kirbyi Honigberg 1955 and Hexamastix mitis sp. nov. Polyphyly of the Monocercomonadidae confirmed that the absence of a costa and an undulating membrane are not taxonomically significant characters and were probably secondarily lost in some or all clades. Our observations, however, indicated that other characters - infrakinetosomal body, comb-like structure, marginal lamella, and the type of axostyle - are fully consistent with the position of Monocercomonadidae species in the parabasalian tree and are, therefore, reasonable taxonomic characters.
References provided by Crossref.org
Molecular evidence of Monocercomonas and Acanthamoeba in the feces of captive reptiles
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