Impedance spectroscopy of bilayer lipid membranes self-assembled on agar support - interaction with HDL
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17552891
DOI
10.33549/physiolres.931305
PII: 1305
Knihovny.cz E-resources
- MeSH
- Agar chemistry MeSH
- Models, Chemical MeSH
- Electric Impedance MeSH
- Electric Capacitance MeSH
- Phosphatidylcholines chemistry MeSH
- Lipid Bilayers * MeSH
- Lipoproteins, HDL chemistry MeSH
- Spectrum Analysis methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Agar MeSH
- Phosphatidylcholines MeSH
- Lipid Bilayers * MeSH
- Lipoproteins, HDL MeSH
The electrical properties of the supported lipid bilayer membrane (s-BLM) of egg phosphatidylcholine (PC) self-assembled on agar surface were examined. To characterize the insulating properties of s-BLMs, electrochemical impedance spectroscopy (EIS) was used. The analysis of impedance spectra in terms of an equivalent circuit of agar/electrolyte and agar/s-BLM/electrolyte in the frequency range of 0.1 Hz-10 kHz was performed. The high-density lipoproteins (HDL)/s-BLM interaction in the concentration range from 20 microg/ml to 80 microg/ml of HDL was investigated. It is evident that treatment of s-BLM with HDL resulted in an increase of the lipid film resistance and a decrease of membrane capacitance.
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