Lack of interference of common phytoecdysteroids with production of nitric oxide by immune-activated mammalian macrophages
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18243265
DOI
10.1016/j.steroids.2007.12.014
PII: S0039-128X(07)00255-3
Knihovny.cz E-resources
- MeSH
- Macrophage Activation drug effects immunology MeSH
- Ecdysteroids chemistry pharmacology MeSH
- Interferon-gamma pharmacology MeSH
- Corticosterone chemistry pharmacology MeSH
- Cells, Cultured MeSH
- Lipopolysaccharides pharmacology MeSH
- Macrophages cytology drug effects immunology MeSH
- Molecular Structure MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Nitric Oxide metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ecdysteroids MeSH
- Interferon-gamma MeSH
- Corticosterone MeSH
- Lipopolysaccharides MeSH
- Nitric Oxide MeSH
Effects of selected common phytoecdysteroids on immunobiological responses triggered by lipopolysaccharide and interferon-gamma (IFN-gamma) were tested under in vitro conditions using murine resident peritoneal macrophages. Namely, production of nitric oxide was investigated. The series of test agents encompassed ecdysteroids occurring often as major components of the ecdysteroid fraction in numerous plant extracts: 20-hydroxyecdysone (20E), polypodine B, ajugasterone C, ponasterone A and inokosterone. Their structural variability concerns only variation in the number and position of hydroxyls. Two additional side-chain modified ecdysteroids: makisterone A (with a methyl substituent at position 24) and carthamosterone (with a cyclic side-chain lactone), and three ecdysteroid analogs: poststerone, rubrosterone and dihydrorubrosterone (devoid of side chains) were included into the test series. All test compounds, except of ponasterone A, represent natural substances isolated from the medicinal plant Leuzea carthamoides and are supposed to be significant for the often reported pharmacological activities of preparations derived from this species. However, the tested ecdysteroids did not interfere with the immunobiological activity of the immunocompetent cells. Our results thus differ from the so far reported information.
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